From I-275 North , take Exit #45-B (I-4) towards Orlando . Follow I-4 for 26 miles. Take Exit #27 FL-570 E toward Lakeland/Winter Haven (portions toll). Then, take Exit 10 to US-98 for nine miles. Merge onto US-17 S. follow for 28 miles to the preserve.
Take US-27 S.- appx 70 miles. Right on SR 64 – appx 12 miles. Turn left on US 64 towards Zolfo Springs – appx 7 miles. Right on US 17 S. –appx ¾ mile.